Coding
Part:BBa_K2686002:Design
Designed by: Thomas Jordan Group: iGEM18_EPFL (2018-09-30)
Encapsulin protein with HexaHistidine insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 492 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457
Design Notes
The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was introduced in between amino acids 43/44 in BBa_K2686001 which is shifted by one amino acid downstream compared to the location in BBa_K192000